Revive Genomics is located in a 112,000 ft2 facility known as the Pennsylvania Biotechnology Center (PA Biotech Center) in Doylestown, PA (Center, http://www.pabiotechbc.org/index.htm). The Center is a recently renovated, $14 million, non-profit business incubator, funded in part by a grant from the Commonwealth of Pennsylvania, whose mission is to nurture entrepreneurship and biotechnology in the region. It is home to 40 technology companies and 2 non-profits, the Blumberg Institute and the Pennsylvania Drug Discovery Institute. The facility provides a shared infrastructure and a collaborative intellectual community allowing for synergistic operations of the companies. Revive has direct access to 750 ft2 laboratory space comprised of separate sections for chemistry and microbiology. It also has a 1000 ft2 minus 20 oC storage facility for extracts and backup samples. Microbial stocks are kept in twelve minus 80 oC Forma freezers. Other assets include five Kuhner cabinets and three roller machines for fermentations. The laboratories are equipped with a biological safety cabinet, Sigma 4-16S table-top centrifuge with holders for 96 well plates, and chemistry fume hoods; they contain an analytical HPLC system (Agilent HP1100), a preparative HPLC setup (complete Gilson 271 system), an Isco Rf200 and miscellaneous sample dryers including a Genevac Tower, rotovaps, low pressure chromatography systems etc. We have access to a Biomek robot for 96-well plate preparation as well as LC/MS and NMR facilities for structural elucidation.
Revive Genomics is currently evaluating the NPDI collection of microbes, comprised of some 36,000 carefully dereplicated and preserved actinomycete and fungal strains collected from around the world. All materials were collected by accredited microbial authorities and in strict adherence to the principles of international biodiversity agreements. The Collection was planned and produced by a team of scientists including both biologists and chemists, allowing for synergy of ideas as well as application of best practices from both disciplines. The microbiology component benefited from long experience in the big Pharma setting in producing secondary metabolites and from lessons learned from decades of screening for medicinally relevant molecules. Empirically optimized media formulations from years of work in both submerged and solid substrate conditions and scalable fermentation processes were the methods of choice for the production of samples.
Shared Resources at the Biotech Center
The Center is equipped with all equipment necessary for modern molecular research. Revive has or has access to via shared services agreements, the following equipment six foot chemical fume hoods, glassware, water baths, rotory evaporators, HPLC, ozonizer and Millipore large scale nanofiltration unit. Additional equipment available at the Biotech Center includes LightCycler LC480 and LightCycler LC2.0 (Roche Biocehmicals), two PCR preparation stations, three Eppendorf gradient thermocyclers, 4 gel electrophoresis system with power suppliers, two refrigerators, two -20 oC freezers,three microcentrifues, Tabletop high speed centrifuges, Micromass ZQ mass Spectrometer with Waters 2695 HPLC with diode array detector, ISCO and Biotage personal chromatography purification equipment, Gilson 215 semi-preparative HPLC system, Biotage Initiator microwave, multiple rotary evaporators, Mettler microbalances, JChem temperature controllers, polarimeter, Innova platform shaker, miscellaneous smaller glassware, PCs, printers, and basic molecular biology related equipment. In addition, Revive has access to liquid scintillation counters, Parr hydrogenator, Perkin Elmer Top Count high throughput plate reader, laminar flow biosafety cabinets, tissue culture incubators, microcentrifuges, icroscopes, centrifuges, ultracentrifuges, TaqMan thermocyclers, Dynal Rotamix, FACS with computer and related software, Thermofinnigan LCQ ion trap mass spectrometer (MALDI-TOF), HPLC with absorbance detector, IR, CD and other common equipment.refrigerators/freezers, PCs, printers, and related equipment.
Collaborative Scientific Environment
Weekly seminars are held within the PA Biotech Center for the discussion of recent results from each laboratory. Weekly meetings are held devoted to the discussion of important journal articles in fields of interest to academic and private business staff. This includes a monthly journal club series including topics on drug discovery, natural products, and computational drug design.